The Hemocytometer blog is the place where you will find everything you need to know on cell counting with a hemocytometer (and some more).
If you’re new to this and you are looking for resources to start with, this is where you should go:
- Hemocytometer protocol: a step by step explanation on how to use the hemocytometer
- Hemocytometer calculation: how to get your cell density once you have your counts
- Calculating dilutions: dilution factors for cell counts
- Hemocytometer protocol – printable sheet!
- Hemocytometer calculation tutorial: video tutorial that walks you through an example calculation of cell density, cell numbers, resuspension volume and cell viability
- Hemocytometer square size: which cells to count where, and what are the areas of the squares you are using
- Counting yeast with a hemocytometer: if you’re counting yeast cells, this one is special for you
- Counting blood cells: same with blood
- Hemocytometer app: a pretty cool iPhone app that does most of the hard work for you
- Cell counting with a compound microscope: 10 step tutorial on how to focus the microscope and use it to count cells
- Starter kit: a list of all the lab materials you need to count cells
If you’re more advanced and you’re wondering what resources related to the hemocytometer you should be using, then head to the specific topics:
- Viability dyes: Trypan blue vs. Erythrosine B
- Quality vs. quantity | Automated cell counter or hemocytometer?
- Hemocytometer app: a pretty cool iPhone app that does most of the hard work for you (this can help a lot experienced people as well!)
For more background on cell culture and where the hemocytometer steps in:
- Cell culture: what it is and how is cell culture classified
- Adherent cells: a brief explanation on how to deattach and subculture them
- Suspension cells: when is the right time to change the medium and how to do it
- Contamination: what types of other things can be growing in your culture that interfere with the cells you are growing
I hope you enjoy the blog! If you have any questions please feel free to leave a comment or send an email to biolabprotocols (at) gmail.com and I will reply asap.