Hemocytometer calculation tutorial

In this tutorial we go through all the calculations explained in hemocytometer calculation but with a small example for both large squares (1mm wide) and small squares (0.2mm wide). We calculate the viability, the cell density, the total number of live cells and the volume to add to reach a target density.

4 comments on “Hemocytometer calculation tutorial

  1. […] can proceed with the counts in the same way as in here, but this time remember to multiply by 200 due to the initial dilution you made, and additionally […]

  2. in regards to the small cells…you said in the tutorial that you counted 5 small squares. Wouldn’t you multiply by the numver of cells you counted. the 0.000004 is for one of the small squares correct?

  3. sorry, disregard previous comment. Revised…in regards to the small squares…you said in the tutorial that you counted 5 small squares. Wouldn’t you multiply by the number of small squares you counted? the 0.000004 is for one of the small squares correct?

    • Hi LeeAnne,
      You take into account the number of squares when taking the average. So you sum the number of cells you have in total among the 5 squares (in this case, 115), you divide by the number of squares (5) and you get your average number of cells per small square. 0.000004 mL is the volume in one small square (inside the central square – see here). Since you have the average number of cells in one small square, you’re good to go!
      Hope that clarifies, let me know otherwise :)

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