I did my PhD in the Department of Chemical Engineering at Imperial College London. My research focused on mathematical modeling of the cell cycle in leukemia and involved experiments with cell lines. During that time, I had to count cells with a hemocytometer so often to track growth that I got tired and decided to build an app, HemocyTap, and share my knowledge on the topic here to help as many people as possible.

Today I’m bringing you a useful dilution factor calculator to help with those quick calculations in the lab. To calculate the dilution factor, you need two things: the original volume of the solution you dilute and the final volume after diluting (or the volume you have added to dilute, in which case the final volume will be the original volume plus the volume you have added). Input those in the two first fields below, and you’ll get the dilution factor and the dilution performed in relative notation. Choose a specific notation from the dropdown box to change it to the standard one you use. See my other post if you need more help carrying out dilutions.


    1. Hi there,

      I’ve put your numbers in and I get 111.11 as a dilution factor:

      This is assuming 45mL is your initial volume and 5L is your final volume. If 5L is the volume you add, then you would put 5.045L in the second box, and would get 112.11 as a dilution factor. Remember to change the units in the dropdown too.

      Did I miss something? Let me know if you have other questions.


  1. Hi,

    thanks Maria for u r reply.

    i need to get dilution factor as 1.67.How ?

    they mentioned as 45 ml of fortune chemical in 5 lit of water. then we will get 1.67 as dilution factor. i don’t know the concentration.

    Can you please help me?

    can you explain

    1. Hi again,

      I don’t really get it as 1.67 would be if you put 3L of the chemical into 2L of water. Are you sure those are the volumes, or are you sure 1.67 is the dilution factor you are seeking? Can you give me a link to the instructions you are following to prepare this solution? I might be able to help further then.


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