In this tutorial we go through all the calculations explained in hemocytometer calculation but with a small example for both large squares (1mm wide) and small squares (0.2mm wide). We calculate the viability, the cell density, the total number of live cells and the volume to add to reach a target density.
I did my PhD in the Department of Chemical Engineering at Imperial College London. My research focused on mathematical modeling of the cell cycle in leukemia and involved experiments with cell lines. During that time, I had to count cells with a hemocytometer so often to track growth that I got tired and decided to build an app, HemocyTap, and share my knowledge on the topic here to help as many people as possible.